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Provedor de dados:  Rev. Microbiol.
País:  Brazil
Título:  Aeromonas species isolated from PINTADO fish (Pseudoplatystoma sp): virulence factors and drug susceptibility
Autores:  Rall,Vera Lúcia Mores
Iaria,Sebastião Timo
Heidtmann,Sandra
Pimenta,Fabiana Cristina
Gamba,Rosa Carvalho
Pedroso,Débora Midori Myaki
Data:  1998-09-01
Ano:  1998
Palavras-chave:  Aeromonas sp
Virulence factors
Drug susceptibility
Fish
Resumo:  Aeromonas has been described as an emergent foodborne pathogen of increasing importance. In this study, we report that 48% of 50 Pintado fish samples collected at the retail market of São Paulo city were positive for Aeromonas sp, as detected by the direct plating method. When the presence/absence method was used, the positivity was 42%. A. caviae was the most frequent species, followed by A. hydrophila and A. sobria. Production of cytotoxic enterotoxin, observed in suckling mouse assay, was detected in 67% of A. sobria strains, in 60% of A. hydrophila strains and in 40% of A. caviae strains. In vitro tests, performed with HEp-2 cells, showed that 88% of A. hydrophila, 27% of A. sobria and 13% of A. caviae strains were positive for this toxin. The in vivo production of cytotonic enterotoxin, tested after heating the filtrates at 56ºC for 20 minutes, was detected in 17% of A. sobria, in 10% of A. caviae and in none of A. hydrophila strains in vivo. All analyzed strains did not alter HEp-2 cells. 20% and 16% of A. sobria and A. caviae isolates, respectively, presented capacity to adhere to HEp-2 cells. In counterpart, invasion of HEp-2 cells was not observed in any isolate. The Aeromonas isolates were sensitive to the majority of the antimicrobiol agents tested.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300015
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S0001-37141998000300015
Formato:  text/html
Fonte:  Revista de Microbiologia v.29 n.3 1998
Direitos:  info:eu-repo/semantics/openAccess
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